Comprehensive transcriptome analysis with the Genome Sequencer FLX System
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چکیده
منابع مشابه
Comprehensive transcriptome analysis with the Genome Sequencer FLX System
Protein isoforms make the transcriptome complex and challenging to resolve. Different forms of a protein may be produced from related genes or may arise from the same gene by alternative splicing. With a combination of long (400–500 bp) 454 SequencingTM reads, dedicated GS De Novo Assembler software and a straightforward protocol using just 200 ng of RNA as sample input, the Genome Sequencer FL...
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Hui Dong1, Yangyi Chen2, Yan Shen2, Shengyue Wang1, Guoping Zhao1,2*, and Weirong Jin1,2* Chinese National Human Genome Center at Shanghai, Shanghai 201203, China National Engineering Center for Biochip at Shanghai, Shanghai 201203, China *Correspondence address. Tel: þ86-21-51320288 (G.Z.)/þ86-21-51320298 (W.J.); Fax: þ86-21-51320288 (G.Z.)/þ86-21-51320298 (W.J.); E-mail: [email protected] (G....
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2011
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.338